Friday, April 20, 2012

MICROBIOLOGY: Lists for practical exam

SLIDES
1.acid fast bacilli TB in sputum(ziehl neelsen stain)
2.candida in culture by gram stain
3.stap.+gram -ve bacilli in culture by gram stain
4.candida+gram -ve bacilli in culture by gram stain
5.aspergillus in SDA by lactophenol cotton blue(LPB)
6.neisseria in urethral discharge by gram stain
7.candida in SDA by LPB
8.septated hypae by LPB
9.non septated hypae by LPB
10.strep.pneumoniae in sputum by gram stain
11.strep.pyogen in sputum by gram stain
12.stap. in pus by gram stain
13.stap. in culture by gram stain.
14.anthracoid in culture by gram stain
15.diphtheroid in culture by gram stain
16.gram -ve bacilli in culture by gram stain
17.anthracoid+gram -ve bacilli in culture by gram stain.

INSTRUMENTS

1.immunofluorescent slide.
2.microtitration plate.
3.bacteriological loop.
4.disposable tissue culture flask(small size)
5.disposable tissue culture flask(large size)
6.disposable tissue culture flask(with tissue culture media)
7.tissue culture bottle
8.tissue culture plate
9.disposable sterile syringe
10.seitz filter
11.blood culture bottle
12.sterile cotton swab
13.disposable sterile container
14.anaerobic jar
15.autoclave

SEROLOGICAL TEST
1.wasserman test
2.widal test.



MEDIA
‎1. Blood agar slope
2. Blood agar plate
3. Deep agar
4. Nutrient agar slope
5. Nutrient agar plate
6. Loeffler's serum
7. LJ medium
8. Peptone water
9. alpha haemolysis on blood agar
10. beta haemolysis on blood agar
11. NLF on Mac Conkey's medium
12. LF on Mac Conkey's medium
13. LF on CLED
14. NLF on CLED
15. Robertson's cooked meat medium
16. Litmus milk
17. Nutrient broth
18. Chocolate agar plate
19. Chocolate agar slope
20. Mac Conkey's medium
21. CLED
22. Dorset egg medium



Cont. :

1. Aspergillus niger on SDA
2. Proteus swarming on nutrient agar plate
3. Candida on SDA
4. Pseudomonas exopigment onnutrient agar
5. Antibiotic sensitivity test (disc diffusion method)
6. Staph. aureus golden yellow endopigment on nutrient agar plate


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